OECD 207
Acute toxicity to earthworms
Test design | |
---|---|
Test species | Eisenia fetida |
Life stage | Adults 2-12 months and 300-600 mg |
Route of exposure | Contact and ingestion |
Endpoints | Mortality (LC50) and biomass changes |
Test duration | 14 days |
Test unit | 500 g equiv. dry soil (750 g wet soil) |
Test concentrations | 5 |
Replicates | 4 |
Specimens | 10/test unit |
Temperature | 20 +/- 2 °C |
Light | Continuous 400-800 lux |
Substrate | Artificial soil |
Treatment application | Even distribution within the soil portion |
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