OECD 203
Fish, Acute Toxicity Test
| Test design | |
|---|---|
| Test species | Brachydanio rerio |
| Life stage | 2.5 cm |
| Route of exposure | Contact and ingestion |
| Endpoints | Mortality (LC50) |
| Test duration | 96 hours |
| Test unit | 4 L of dilution water |
| Test concentrations | 5 |
| Replicates | 1 |
| Specimens | 7/test unit |
| Temperature | 21-25 +/- 2 °C |
| Light | 16h Light <700lux / 8h Dark |
| Substrate | Dilution water: ISO6341 |
| Treatment application | Spiked water |
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