OECD 222
Earthworm reproduction test
| Test design | |
|---|---|
| Test species | Eisenia fetida |
| Life stage | Adults 2-12 months and 250-600 mg |
| Route of exposure | Contact and ingestion |
| Endpoints | Reproductive output (EC50) and biomass changes |
| Test duration | 8 weeks |
| Test unit | 500 g equiv. dry soil (750 g wet soil) |
| Test concentrations | 8 |
| Replicates | 4 |
| Specimens | 10/test unit |
| Temperature | 20 +/- 2 °C |
| Light | 16h light(400-800 lux)/8h night |
| Substrate | Artificial soil |
| Treatment application | Even distribution within the soil portion |
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